Methods By means of bicolour fluorescence and flow cytometry (FCM) analysis, the results were compared during different lay-out time, hemolysis time and washing time.

方法用抗免疫荧光标记，流式细胞分析，对标本放置不同时、后洗涤前后的结果进行比较。

Conclusion:The purified fusion protein has good fineness and hemolysis activity, the antibody titers initiated by the protein vaccine go with regulation and the immunoprotection is satisfied.

纯化的目的蛋白达到电泳级纯度，且依然保持良好的黏附活性，蛋白疫苗作用机产生的抗水平符合抗消长规律。